Through screening multiple resistant calli and resistant regenerated seedlings， we obtained regenerated transgenic plants expressing each vector.

L -1 acetosyringone and 0.01% Silwet L-77， followed by vacuum treatment for 10 mins， then soaking for 30 mins and co-culturing for 2 days.The optimal transformation conditions were as follows： ultrasonic treatment of the explant for 20 min with bacterial solution （concentration OD 600=0.6） containing 100 μmol Calli subcultured for 24 weeks were selected for Agrobacterium-mediated genetic transformation. The optimal concentration of glufosinate for screening was 1.2 mg The optimal concentration of hygromycin for screening was 120 mg The results showed that the regeneration rate of callus decreased significantly after subculture for 36 weeks， and the highest regeneration rate was 67.9% after subculture for 48 weeks. After optimizing the genetic transformation conditions， we compared the transformation efficiency of hpt and bar genes as selection markers in Agrobacterium-mediated transformation of seashore paspalum.

To establish an efficient and stable genetic transformation system for this plant， we used its mature seeds as explants， and determined the optimal selection pressures of hygromycin and glufosinate during callus subculture and the regeneration stage. Seashore paspalum is a warm-season turfgrass with strong salt tolerance， and it has a wide range of potential applications.